The cell line was established by transfection using a pta nfkb luciferase reporter vector which contains 4 repeats of nfkb binding sites a minimal promoter upstream of the firefly luciferase coding region along with hygromycin expression vector followed by hygromycin selection. The tlr2 nf kb leeporter luciferase reporter cell line is a stably transfected hek 293 cell line which expresses full length human toll like receptor 2 tlr2 and renilla luciferase reporter gene under the transcriptional control of the nf kb response element. Thp1 lucia cells feature the lucia gene a secreted luciferase reporter gene driven by an ifn β minimal promoter fused to five copies of the nf κb consensus transcriptional response element and three.
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